Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ FastDigest XapI
FastDigest XapI restriction enzyme recognizes R^AATTY sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AcsI, ApoI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | XapI |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 80°C |
| Product Line | FastDigest |
Thermo Scientific™ Alw21I (BsiHKAI) (10 U/μL)
Cut at GWGCW^C sites with Alw21I (BsiHKAI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: Bbv12I, BsiHKAI).
Thermo Scientific™ FastDigest TauI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest SatI
Cut at GC^NGC sites with Thermo Scientific™ FastDigest™ Fnu4HI (SatI), which performs best using universal FastDigest Buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI).
Thermo Scientific™ CaiI (AlwNI) (10 U/μL)
Cut at CAGNNN^CTG sites with CaiI (AlwNI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AlwNI).
Thermo Scientific™ Hpy8I (MjaIV) (10 U/μL)
The Hpy8I (MjaIV) restriction enzyme recognizes GTN^NAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: Hpy166II).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Hpy8I (MjaIV) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ BseJI (BsaBI) (10 U/μL)
Cut at GATNN^NNATC sites with BseJI (BsaBI) restriction enzyme, which performs best at 65°C in O buffer (Isoschizomers: BsaBI, Bse8I).
Thermo Scientific™ FastDigest FokI
Cut at GGATG(N)9^ sites with Thermo Scientific™ FastDigest™ FokI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest BmsI (IIs class)
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ CseI (HgaI) (5 U/μL)
Cut at GACGC(5/10)^ sites with CseI (HgaI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: HgaI).
Thermo Scientific™ Ecl136II, PacI, SacI Buffer (10X)
Optimize digestions with Ecl136II, PacI, SacI restriction enzymes using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ Buffer O (10X)
Ensure the optimum reaction conditions for restriction enzymes with 10X Buffer O, premixed with BSA for enhanced stability.
Thermo Scientific™ EheI (SfoI) (10 U/μL)
Cut at GGC^GCC sites with EheI (SfoI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
Thermo Scientific™ HincII (HindII) (10 U/μL)
The HincII (HindII) restriction enzyme recognizes GTY^RAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: HindII).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | HincII (HindII) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ BcnI (NciI) (10 U/μL)
Cut at CC^SGG sites with BcnI (NciI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AsuC2I, BpuMI, NciI).